thiM [2019-08-12 12:34:48]

hydroxyethylthiazole kinase

locus: BSU_38300

pI: 5.39

mw: 28.06 kDa

protein length: 272 aa

gene length: 819 bp

function: biosynthesis of thiamine

product: hydroxyethylthiazole kinase

essential: no

ec: 2.7.1.50

synonyms: thiK,ipa-25d,ywbJ

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.2|Biosynthesis of cofactors] → [category|SW 2.6.2.3|Biosynthesis/ acquisition of thiamine]

Gene

Coordinates: 3,931,372 3,932,190

The protein

Catalyzed reaction/ biological activity

• ATP + 4-methyl-5-(2-hydroxyethyl)thiazole = ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole (according to Swiss-Prot)

Protein family

• Thz kinase family (single member, according to UniProt)

Structure

• [PDB|1C3Q]

Expression and Regulation

Operons

• genes: [gene|CC55120C2AF455FA2AEAE39E21F57897B2B5BC39|ywbI]-[gene|9B82059498F72A67282DD54892EF02A305E3C674|thiM]-[gene|8FAEB6342E087BEDC16FDF5585B03B90ACFF8D11|thiE]
  description: [Pubmed|9139923]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|9139923], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

Biological materials

Mutant

• BKE38300 ([gene|9B82059498F72A67282DD54892EF02A305E3C674|thiM]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE38300 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATTTGCATCATCCTTTA, downstream forward: _UP4_TCATGACTCGTATTTCTCGG
• BKK38300 ([gene|9B82059498F72A67282DD54892EF02A305E3C674|thiM]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK38300 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATTTGCATCATCCTTTA, downstream forward: _UP4_TCATGACTCGTATTTCTCGG

References

Reviews

• 19348578

Original publications

• 10891066,9139923